Bioactivity-guided fractionation and GC/MS fingerprinting of Angelica sinensis and Angelica archangelica root components for antifungal and mosquito deterrent activity
Autor(es): Wedge, David E.; Klun, Jerome A.; Tabanca, Nurhayat; Demirci, Betul; Ozek, Temel; Baser, Kemal Husnu Can; Liu, Zhijun; Zhang, Sui; Cantrell, Charles L.; Zhang, Jian
Resumo: Bioassay-guided fractionation of the chloroform extract from the roots of Angelica sinensis led to isolation and characterization of (Z)-ligustilide using direct-bioautography with Colletotrichum species. The structure of (Z)-ligustilide was confirmed by (1)H and (13)C NMR spectroscopy and GC/MS. (Z)-Ligustilide deterred the biting of two mosquito species more effectively than DEET. Three different A. sinensis accessions and one Angelica archangelica root oil were evauated by GC and GC/MS, and the dominant component in A. sinensis was 61-69% (Z)-ligustilide. Two other prominent compounds in A. sinensis oils were 5.7-9.8% (E)-3-butylidene phthalide and 1.5-2.3% (Z)-3-butylidene phthalide. The main constituents that comprised A. archangelica oil were monoterpene hydrocarbons such as 24.5% alpha-pinene, 13.8% delta-3-carene, 10.1% beta-phellandrene, 8.8% p-cymene, 8.4% limonene, and 6.3% sabinene. Phthalides and monoterpene hydrocarbons were determined to be good systematic markers or chemical fingerprints for A. sinensis and A. archangelica root oils. Chemical fingerprinting by GC/MS of A. sinensis also confirmed the misidentification of one A. archangelica sample sold in the Chinese market.
Palavras-Chave: (Z)-Ligustilide; Aedes aegypti; Anopheles stephensi; Colletotrichum acutatum; Colletotrichum fragariae; Colletotrichum gloeosporioides; Dang gui; Dong quai
Imprenta: Journal of Agricultural and Food Chemistry, v. 57, n. 2, p. 464-470, 2009
Identificador do objeto digital: 10.1021/jf802820d
Descritores: Aedes aegypti - Repellent
Data de publicação: 2009