Isolation and molecular characterization of Chikungunya virus from the Andaman and Nicobar archipelago, India: evidence of an East, Central, and South African genotype
Autor(es): Muruganandam, N.; Chaaithanya, I. K.; Senthil, G. S.; Shriram, A. N.; Bhattacharya, D.; JeevaBharathi, G. S.; Sudeep, A. B.; Pradeep Kumar, N.; Vijayachari, P.
Resumo: Chikungunya virus (CHIKV) is an Alphavirus belonging to the family Togaviridae. In 2006, CHIKV infection struck the Andaman and Nicobar archipelago, with an attack rate of 60%. There were more than 10 cases with acute flaccid paralysis simulating the Guillian Barre Syndrome. The majority of the patients presented severe joint pain. The cause for such an explosive nature of the outbreak with increased morbidity was not known. The isolation of CHIKV was attempted and succeeded from nine subjects presenting clinical symptoms of Chikungunya fever. The cDNA of all the isolates was sequenced for partial E1 and nsP1 genes. Sequences were aligned based on the double locus sequence typing concept. The phylogenetic analysis shows that sequences of Andaman isolates grouped with the East, Central, and South African genotype of virus isolates from India, Sri Lanka, and Reunion. The genetic distance between Andaman isolates and the Reunion isolates was very small. The phylogenetic analysis confirmed the origin of the isolates responsible for the first ever confirmed CHIKV outbreak in these islands to be the East, Central, and South African genotype. In this manuscript, we discuss the involvement of the East, Central, and South African strain with the Chikungunya fever outbreak in this archipelago and double locus sequence typing as a first time approach.Original Abstract: Le virus Chikungunya (CHIKV) est un Alphavirus appartenant a la famille des Togaviridae. En 2006, une infection au CHIKV a frappe l'archipel Andaman-et-Nicobar, avec un taux d'atteinte de 60 %. Plus de 10 cas presentant une paralysie flasque simulant le Syndrome de Guillain-Barre ont ete rapportes. La majorite des patients presentaient des douleurs articulaires severes. La cause d'une eclosion presentant un caractere si violent accompagne d'une morbidite elevee n'etait pas connue. L'isolement de CHIKV a ete tente et reussi a partir de neuf sujets presentant des symptomes cliniques de la fievre a CHIKV. L'ADNc de tous les isolats a ete sequence partiellement au niveau des genes E1 et nsP1. Les sequences ont ete alignees selon le concept du typage de sequence double locus (DLST). L'analyse phylogenique a montre que les sequences des isolats d'Andaman etaient groupees avec le genotype ECSA des isolats viraux des souches de l'Inde, du Sri Lanka et des Iles de la Reunion. La distance genetique entre les isolats d'Andaman et la souche de la Reunion etait tres courte. L'analyse phylogenique a confirme que l'origine des isolats responsables de la premiere eclosion de CHIKV jamais confirmee dans ces iles est du genotype ECSA. Dans ce manuscrit, nous discutons de l'implication de la souche ECSA dans l'eclosion de la fievre Chikungunya dans cet archipel et du DLST comme approche utilisee pour la premiere fois.
Palavras-Chave: Symptoms; Nucleotide sequence; Microbiology; Archipelagoes; Genotypes; Phylogenetics; Paralysis; Phylogeny; Fever; Islands; Pain; Explosives; Genetic distance; Infection; Morbidity; Joints; Chikungunya virus; Alphavirus; Togaviridae
Imprenta: Canadian Journal of Microbiology/Revue Canadienne de Microbiologie, v. 57, n. 12, p. 1073-1077, 2011
Descritores: Guillain-Barre Syndrome and Molecular Structure ; Guillain-Barre Syndrome and Virus ; Guillain-Barre Syndrome and Chikungunya Fever ; Guillain-Barre Syndrome and Public health
Data de publicação: 2011
