West Nile virus infection alters midgut gene expression in Culex pipiens quinquefasciatus say (Diptera: Culicidae)
Autor(es): Smartt, C. T.; Richards, S. L.; Anderson, S. L.; Erickson, J. S.
Resumo: Alterations in gene expression in the midgut of female Culex pipiens quinquefasciatus exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding on infected blood. Of these, 21 cDNAs showed an increase in expression, and 5 showed a decrease in expression as a result of WNV presence in the blood meal. GenBank database searches showed that one clone with increased expression, CQ G12A2, shares 94% identity with a leucine-rich repeat-containing protein from Cx. p. quinquefasciatus and 32% identity to Toll-like receptors from Aedes aegypti. We present the first cDNA clone isolated from female Cx. p. quinquefasciatus midgut tissue whose expression changes on exposure to WNV. This cDNA represents a mosquito gene that is an excellent candidate for interacting with WNV in Cx. p. quinquefasciatus and may play a role in disease transmission.
Palavras-Chave: Mosquitoes; Gene expression; Viral diseases; Aquatic insects; Disease transmission; Public health; Databases; Blood; Feeding; Blood meals; Midgut; Infection; Differential display; Toll-like receptors
Imprenta: American Journal of Tropical Medicine and Hygiene, v. 81, n. 2, 2009.
Descritores: Aedes aegypti - Proteins ; Aedes aegypti - Viral infections ; Aedes aegypti - Virus ; Aedes aegypti - Transmission ; Aedes aegypti - Public health
Data de publicação: 2009
