Autor(es): Edwards, M. J.; Severson, D. W.; Hagedorn, H. H.

Resumo: Vitelline envelope genes from the mosquito Aedes aegypti were analyzed with respect to their DNA sequences, genomic representation, temporal and spatial expression profiles and response to 20-hydroxyecdysone. Genomic clones of three vitelline envelope genes, 15a-1, 15a-2 and 15a-3 were isolated. Southern analysis indicates that all three genes are represented by a single copy in the genome. The deduced amino acid sequences of all three vitelline envelope genes contain a conserved region of 46 residues that overlaps with a region that is conserved in four Drosophila melanogaster vitelline envelope genes. DNA was sequenced flanking the 15a-1, 15a-2 and 15a-3 coding regions. A 360 bp sequence 5 theta of the 15a-2 coding region was identified with 72% identity to a sequence upstream of the Ae. aegypti VgA1 vitellogenin gene. The temporal patterns of 15a-1, 15a-2 and 15a-3 expression, as determined by Northern analysis, were similar. The spatial patterns of expression, as determined by whole-mount in situ hybridization, differed between the three genes. 15a-1 and 15a-3 were only expressed in the middle and posterior regions of the follicle, while 15a-2 was also expressed at the anterior region. Vitelline envelope gene expression was higher in ovaries that were dissected at 0, 2 and 10 h following a blood meal and then incubated in vitro for 10 h in medium containing 10 super(-5) M 20-hydroxyecdysone, compared to ovaries that were incubated without hormone.

Palavras-Chave: Mosquitoes; Yellow fever mosquito; Aedes aegypti; Vitelline envelope; 20-hydroxyecdysone

Imprenta: Insect Biochemistry and Molecular Biology, v. 28, n. 12, p. 915-925, 1998.

Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Molecular Structure

Data de publicação: 1998

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