Use of centrifugal filter devices to concentrate dengue virus in mosquito per os infection experiments
Autor(es): Richard, Vaea; Viallon, Jerome; Cao-Lormeau, Van-Mai
Resumo: Dengue virus (DENV) is an arbovirus transmitted to humans by the bite of infected Aedes mosquitoes. Experimental per os infection of mosquitoes with DENV is usually a preliminary step in virus/vector studies but it requires being able to prepare artificial blood-meals with high virus titers. We report here the convenient use of centrifugal filter devices to quickly concentrate DENV particles in cell-culture supernatants. The median viral titer in concentrated-supernatants was 8.50 log10 TCID50/mL. By using these DENV concentrated-supernatants to prepare infectious blood-meals in Aedes aegypti per os infection experiments, we obtained a mean mosquito-infection rate of 94%. We also evaluated the use of centrifugal filter devices to recover DENV particles from non-infectious blood-meals presented to infected mosquitoes through a feeding membrane to collect their saliva.
Palavras-Chave: Human diseases; Viral diseases; Hosts; Aquatic insects; Disease transmission; Public health; Filters; Feeding; Bites; Vectors; Saliva; Infection; Dengue virus; Aedes aegypti; Arbovirus
Imprenta: Plos One, v. 10, n. 9, 2015.
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Cell ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Viral infections ; Aedes aegypti - Virus ; Aedes aegypti - Transmission ; Aedes aegypti - Dengue
Data de publicação: 2015
