Towards a semen proteome of the dengue vector mosquito: protein identification and potential functions
Autor(es): Sirot, Laura K.; Hardstone, Melissa C.; Helinski, Michelle E. H.; Ribeiro, Jose M. C.; Kimura, Mari; Deewatthanawong, Prasit; Wolfner, Mariana F.; Harrington, Laura C.
Resumo: Dengue is a potentially lethal infection that impacts millions of humans annually. This disease is caused by viruses transmitted by infected female Aedes aegypti mosquitoes during blood feeding. No commercial vaccine or treatment is available for dengue infection. One way to break the disease transmission cycle is to develop new tools to reduce dengue vector populations. Seminal fluid proteins (Sfps) produced in the reproductive glands of male mosquitoes and transferred to females in the ejaculate during mating could be the target of such a tool. In related insects, Sfps modulate female reproduction and feeding patterns. Here we report 145 proteins that are transferred to females in the Ae. aegypti ejaculate. The proteins, which include Sfps and sperm proteins, fall into biochemical classes that suggest important potential roles in mated females. Of particular interest are proteins that could play roles in fertility and hormonal activity (including pathways involved in egg development and utilization of the blood meal). Our results lay important groundwork for new control strategies by identifying candidate proteins that may alter the reproductive biology or blood-feeding patterns of female Ae. aegypti and ultimately reduce the global burden of dengue.
Palavras-Chave: Human diseases; Glands; Viruses; Disease control; Pest control; Hosts; Aquatic insects; Disease transmission; Public health; Feeding; Fertility; Vectors; Blood meals; Sperm; Infection; Mating; Blood; Dengue; Semen; Reproduction; Vaccines; Seminal fluid; Aedes aegypti
Imprenta: Plos Neglected Tropical Diseases, v. 5, n. 3, 2011.
Descritores: Aedes aegypti - Proteins ; Aedes aegypti - Proteome ; Aedes aegypti - Virus ; Aedes aegypti - Transmission ; Aedes aegypti - Dengue ; Aedes aegypti - Public health ; Aedes aegypti - Vaccine
Data de publicação: 2011
