Autor(es): Figueira-Mansur, J.; Ferreira-Pereira, A.; Mansur, J. F.; Franco, T. A.; Alvarenga, E. S. L.; Sorgine, M. H. F.; Neves, B. C.; Melo, A. C. A.; Leal, W. S.; Masuda, H.; Moreira, M. F.

Resumo: Re-emergence of vector-borne diseases such as dengue and yellow fever, which are both transmitted by the Aedes aegypti mosquito, has been correlated with insecticide resistance. P-glycoproteins (P-gps) are ATP-dependent efflux pumps that are involved in the transport of substrates across membranes. Some of these proteins have been implicated in multidrug resistance (MDR). In this study, we identified a putative P-glycoprotein in the Ae. aegypti database based on its significantly high identity with Anopheles gambiae, Culex quinquefasciatus, Drosophila melanogaster and human P-gps. The basal ATPase activity of ATP-binding cassette transporters in larvae was significantly increased in the presence of MDR modulators (verapamil and quinidine). An eightfold increase in Ae. aegypti P-gp (AaegP-gp) gene expression was detected in temephos-treated larvae as determined by quantitative PCR. To analyse the potential role of AaegP-gp in insecticide efflux, a temephos larvicide assay was performed in the presence of verapamil. The results showed an increase of 24% in temephos toxicity, which is in agreement with the efflux reversing effect. RNA interference (RNAi)-mediated silencing of the AaegP-gp gene caused a significant increase in temephos toxicity (57%). In conclusion, we have demonstrated for the first time in insects that insecticide-induced P-gp expression can be involved in the modulation of insecticide efflux.

Palavras-Chave: Gene expression; Molecular biology; Insecticides; Insect larvae; Pest control; Toxicity; Aquatic insects; Mortality causes; Public health; Mortality; Adenosinetriphosphatase; Vector-borne diseases; Disease transmission; Databases; P-Glycoprotein; Verapamil; Dengue; ATP-binding protein; Yellow fever; Quinidine; RNA-mediated interference; Polymerase chain reaction; Multidrug resistance; Larvicides; Culex quinquefasciatus; Aedes aegypti; Drosophila melanogaster; Anopheles gambiae

Imprenta: Insect Molecular Biology, v. 22, n. 6, p. 648-658, 2013.

Descritores: Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - Transmission ; Aedes aegypti - Larvicide ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2013

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