In vitro expression and pharmacology of the 5-HT sub(7)-like receptor present in the mosquito Aedes aegypti tracheolar cells and hindgut-associated nerves
Autor(es): Lee, D.; Pietrantonio, P. V.
Resumo: We have previously reported the cloning of a 5-hydroxytryptamine receptor (Aedes 5-HT sub(7)-like receptor) from adult Aedes aegypti. For functional expression of the Aedes 5-HT sub(7)-like receptor, CHO-K1 cells were stably transfected with a receptor expression construct, pC5-HT sub(7). The Aedes 5-HT sub(7)-like receptor positively coupled to Gs protein, increasing intracellular cAMP in response to 5-HT; adenylyl cyclase activity was induced in a concentration-dependent, saturable manner. Only 5-HT, and not octopamine, dopamine or tyramine, caused the induction of cAMP. At 10 nm 5-HT a weak synergism was observed between octopamine and 5-HT. Other known agonists of the mammalian 5-HT sub(7) receptor were tested. Their order of potency was: 5-HT >> 5-CT = 8-OH-DPAT >> pimozide. This is the first report on the functional expression of a mosquito neurohormone receptor.
Palavras-Chave: Diptera; Mosquitoes; Yellow fever mosquito; Octopamine; Tyramine
Imprenta: Insect Molecular Biology, v. 12, n. 6, p. 561-569, 2003.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Proteins
Data de publicação: 2003
