Autor(es)Caragata, E. P.; Poinsignon, A.; Moreira, L. A.; Johnson, P. H.; Leong, Y. S.; Ritchie, S. A.; O'Neill, S. L.; McGraw, E. A.

ResumoTranscriptional profiling is an effective method of predicting age in the mosquito Aedes aegypti in the laboratory, however, its effectiveness is limited to younger mosquitoes. To address this we used a microarray to identify new gene candidates that show significant expression changes in older mosquitoes. These genes were then used to create a revised model, which upon evaluation in both laboratory and semi-field conditions, proved to have improved accuracy overall and for older mosquitoes. In association with the development of symbiont-based control strategies for Ae. aegypti, we also tested the model's accuracy for Wolbachia-infected mosquitoes and found no decline in performance. Our findings suggest that the new model is a robust and powerful tool for age determination in Australian Ae. aegypti populations.

Palavras-ChaveMolecular biology; Grading; Profiling; Pest control; Age determination; Aquatic insects; Cages; Age; Transcription; Infection; DNA microarrays; Models; Aedes aegypti; Wolbachia

ImprentaInsect Molecular Biology, v. 20, n. 2, p. 215-224, 2011.

DescritoresAedes aegypti - DNA ; Aedes aegypti - Molecular methods

Data de Publicação:2011