Autor(es): Sharakhova, Maria V.; Timoshevskiy, Vladimir A.; Yang, Fan; Demin, Sergei Iu; Severson, David W.; Sharakhov, Igor V.

Resumo: Dengue fever is an emerging health threat to as much as half of the human population around the world. No vaccines or drug treatments are currently available. Thus, disease prevention is largely based on efforts to control its major mosquito vector Ae. aegypti. Novel vector control strategies, such as population replacement with pathogen-incompetent transgenic mosquitoes, rely on detailed knowledge of the genome organization for the mosquito. However, the current genome assembly of Ae. aegypti is highly fragmented and requires additional physical mapping onto chromosomes. The absence of readable polytene chromosomes makes genome mapping for this mosquito extremely challenging. In this study, we discovered and investigated a new source of chromosomes useful for the cytogenetic analysis in Ae. aegypti - mitotic chromosomes from imaginal discs of 4th instar larvae. Using natural banding patterns of these chromosomes, we developed a new band-based approach for physical mapping of DNA probes to the precise chromosomal positions. Further application of this approach for genome mapping will greatly enhance the utility of the existing draft genome sequence assembly for Ae. aegypti and thereby facilitate application of advanced genome technologies for investigating and developing novel genetic control strategies for dengue transmission.

Palavras-Chave: DNA; Disease control; Pest control; Hosts; Aquatic insects; Public health; Disease transmission; Polytene chromosomes; Imaginal discs; DNA probes; Nucleotide sequence; Vectors; Dengue; Yellow fever; Physical mapping; Genetic control; Vaccines; Drugs; Gene mapping; Aedes aegypti

Imprenta: Plos Neglected Tropical Diseases, v. 5, n. 10, 2011.

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Transmission ; Aedes aegypti - Vaccine ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2011

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