Autor(es): Baron, Olga L.; Ursic-Bedoya, Raul J.; Lowenberger, Carl A.; Ocampo, Clara B.

Resumo: Suppressive subtractive hybridization was used to evaluate the differential expression of midgut genes of feral populations of Aedes aegypti (Diptera: Culicidae) from Colombia that are naturally refractory or susceptible to Dengue-2 virus infection. A total of 165 differentially expressed sequence tags (ESTs) were identified in the subtracted libraries. The analysis showed a higher number of differentially expressed genes in the susceptible Ae. aegypti individuals than the refractory mosquitoes. The functional annotation of ESTs revealed a broad response in the susceptible library that included immune molecules, metabolic molecules and transcription factors. In the refractory strain, there was the presence of a trypsin inhibitor gene, which could play a role in the infection. These results serve as a template for more detailed studies aiming to characterize the genetic components of refractoriness, which in turn can be used to devise new approaches to combat transmission of dengue fever.

Palavras-Chave: Gene expression; Bibliographic information; Population genetics; Human diseases; Transcription; Pest control; Hybridization; Public health; Dengue; Transcription factors; Midgut; Infection; Feral populations; Aedes aegypti; Culicidae; Dengue virus type 2

Imprenta: Journal of Insect Science, v. 10, n. 41, p. 1-23, 2010.

Descritores: Aedes aegypti - Immune response ; Aedes aegypti - virus ; Aedes aegypti - Transmission ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2010