Autor(es): Olson, K. E.; Myles, K. M.; Seabaugh, R. C.; Higgs, S.; Carlson, J. O.; Beaty, B. J.

Resumo: A double subgenomic Sindbis (dsSIN) virus, MRE/3'2 J/GFP, was constructed to efficiently express green fluorescent protein (GFP) in the midgut of Aedes aegypti following per os infection. The MRE/3'2 J/GFP RNA genome contained the nonstructural genes and cis-acting sequences of the dsSIN virus, TE/3'2 J/GFP, but had the structural genes of MRE16 SIN virus. MRE/3'2 J/GFP virus, unlike TE/3'2 J/GFP virus, efficiently infected mosquitoes orally. At 1-2 days postinfection, GFP was observed as multiple foci of expression on the lumenal side of the midgut. At 10-12 days post-infection, thirteen of fifteen mosquitoes infected with MRE/3'2 J/GFP virus had high levels of GFP expression in the mosquito midgut. The MRE3'2 J dsSIN expression system should be an important tool for efficient gene expression in Ae. aegypti midguts.

Palavras-Chave: Protein synthesis; Genes; Digestive system; Gene expression; Gene regulation; Green fluorescent protein

Imprenta: Insect Molecular Biology, v. 9, n. 1, p. 57-65, 2000.

Descritores: Aedes aegypti - Protein synthesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Virus

Data de publicação: 2000