Detection of dengue virus from field Aedes aegypti and Aedes albopictus adults and larvae
Autor(es): Ahmad, R.; Ismail, A.; Saat, Z.; Lim, Lee Han
Resumo: Mosquito adults and larvae were collected from dengue high risk areas and transported to the laboratory for identification. Identified mosquitos were pooled according to the species, date and locality and stored at -70 degree C. A total of 1,385 pools of Aedes albopictus and 267 pools of Ae. aegypti were collected from major towns in 12 states in Peninsular Malaysia. Virus isolation was carried out using cell culture (C6/36 clone) of Ae. albopictus and detection of dengue virus by the peroxidase anti-peroxidase staining. All positive isolations were further re-confirmed by the reverse transcriptase-polymerase chain reaction (RT-PCR). Most of the pools were negative with PAP staining and RT-PCR. However, 11 mosquito pools were positive with PAP staining. On the other hand, samples from Terengganu, Pulau Pinang and Johor were positive using both methods.
Palavras-Chave: Biological vectors; Human diseases; Viral diseases; Insect larvae; DNA; Disease detection; Aquatic insects; Public health; Aedes aegypti; Culicidae; Aedes albopictus
Imprenta: Southeast Asian Journal of Tropical Medicine & Public Health, v. 28, n. 1, p. 138-142, 1997.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Viral infections ; Aedes aegypti - PCR detection ; Aedes aegypti - RT-PCR ; Aedes aegypti - Virus ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 1997
