Deltamethrin resistance mechanisms in Aedes aegypti populations from three french overseas territories worldwide
Autor(es): Dusfour, Isabelle; Zorrilla, Pilar; Guidez, Amandine; Issaly, Jean; Girod, Romain; Guillaumot, Laurent; Robello, Carlos; Strode, Clare
Resumo: Aedes aegypti is vector of Dengue, Chikungunya and Zika viruses, all causing emerging or re-emerging diseases worldwide. Fighting these diseases relies on the control of the vector. Therefore, insecticides have been extensively used worldwide, resulting in the development of insecticide resistance. In the French overseas territories, resistance to pyrethroids has been monitored for many years with high levels in the South American French territories. We then investigated the mechanisms underlying this resistance in populations from French Guiana, Guadeloupe and New Caledonia. Transcription levels of detoxification genes were measured and alongside screening for target site mutations. Upregulation of cytochrome P450 genes and carboxylesterases were observed in all three populations. Mutations related to pyrethroid resistance in position 1016 and 1534 of the voltage-gated sodium channel gene were also observed. French Guiana and Guadeloupe populations presented a closer profile of resistance mechanisms whereas the New Caledonia population had a more restricted profile. Such differences can be explained by different vector control practices, regional insecticide uses and genetic backgrounds. These results are also compared with others obtained from other parts of the world and are discussed with the perspective of integrative research on vector competence.
Palavras-Chave: Pest control; Home range; Hosts; Resistance mechanisms; Public health; Disease transmission; Detoxification; Vectors; Carboxylesterase; Transcription; Territory; Deltamethrin; Dengue; Cytochrome P450; Pyrethroids; Mutation; Aedes aegypti
Imprenta: Plos Neglected Tropical Diseases, v. 9, n. 11, 2015.
Descritores: Aedes aegypti - Virus ; Aedes aegypti - Transmission ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2015
