Construction and characterization of the interdomain chimeras using Cry11Aa and Cry11Ba from Bacillus thuringiensis and identification of a possible novel toxic chimera
Autor(es): Sun, Yunjun; Zhao, Qiang; Zheng, Dasheng; Ding, Xuezhi;Wang, Jingfang; Hu, Quanfang; Yuan, Zhiming; Park, Hyun-Woo; Xia, Liqiu
Resumo: Three structural domains of mosquitocidal Cry11Aa and Cry11Ba from Bacillus thuringiensis were exchanged to produce interdomain chimeras [BAA (11Ba/11Aa/11Aa), ABA (11Aa/11Ba/11Aa), AAB (11Aa/11Aa/11Ba), ABB (11Aa/11Ba/11Ba), BAB (11Ba/11Aa/11Ba), BBA (11Ba/11Ba/11Aa]. Chimeras BAB, BAA, BBA, and AAB formed inclusion bodies in the crystal-negative B. thuringiensis host and produced expected protein bands on SDS-PAGE gel. However, no inclusion body or target protein could be found for chimeras ABA and ABB. In bioassays using the fourth-instar larvae of Culex quinquefasciatus and Aedes aegypti, AAB had 50 % lethal concentrations of 4.8 and 2.2 mu g ml super(-1), respectively; however, the rest of chimeras were not toxic. This study thus helps to understand the domain-function relationships of the Cry11Aa and Cry11Ba toxins. The toxic chimera, AAB, might be a candidate for mosquito control as its amino acid sequence is different from the two parental toxins.
Palavras-Chave: Biological control; Bioassays; Lethal limits; Toxicants; DNA; Pest control; Aquatic insects; Biotechnology; Public health; Chimeras; Inclusion bodies; Toxins; Amino acid sequence
Imprenta: Biotechnology Letters, v. 36, n. 1, p. 105-111, 2014.
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Proteins ; Aedes aegypti - Public health
Data de publicação: 2014
