Comparative linkage maps for the mosquitoes (Culex pipiens and Aedes aegypti) based on common RFLP Loci
Autor(es): Mori, A.; Severson, D. W.; Christensen, B. M.
Resumo: We report construction of a comparative linkage map for the mosquito (Culex pipiens) based on restriction fragment length polymorphisms (RFLPs) using cDNA clones from Aedes aegypti as probes to Southern blots of Cx. pipiens genomic DNA. Seventy-one cDNA clones were screened for hybridization and genetic diversity among three Cx. pipiens strains. Fifty-two of 71 cDNA clones, isolated from and previously mapped in Ae. aegypti (73.2%), were hybridized under high-stringency conditions with Cx. pipiens genomic DNA. Thirty-four clones (47.9%) reflected strain-specific polymorphisms. The map consists of 21 cDNA markers that identify 22 loci covering 165.8 cM. The loci mapped in Cx. pipiens cover 7.1 cM on chromosome 1, 80.4 cM on chromosome 2, and 78.3 cM on chromosome 3. Linkage relationships of the RFLP markers for chromosome 1 in Cx. pipiens are the same as chromosome 1 in Ae. aegypti, indicating that chromosome 1 is highly conserved between the two species. The comparative RFLP linkage maps for chromosomes 2 and 3 in Cx. pipiens and Ae. aegypti reflect apparent whole-arm translocations. RFLP markers for chromosome 2 in Ae. aegypti identified homologous loci on one arm of chromosomes 2 and 3 in Cx. pipiens, and RFLP markers for chromosome 3 in Ae. aegypti identified homologous loci on the opposite arms of chromosomes 2 and 3 in Cx. pipiens.
Palavras-Chave: Molecular structure; Genomes; Chromosomes; Genes; Biopolymorphism; Aquatic insects; Linkage analysis; Restriction fragment length polymorphism; Gene mapping
Imprenta: Journal of Heredity, v. 90, n. 1, p. 160-164, 1999.
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Molecular Structure
Data de publicação: 1999
