Cloning and expression of two crystal protein genes, cry30Ba1 and cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus INA288
Autor(es): Ito, Takeshi; Ikeya, Tomonori; Sahara, Ken; Bando, Hisanori; Asano, Shin-ichiro
Resumo: Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC sub(50)] = 6 ng/ml) and Aedes aegypti (LC sub(50) = 12 ng/ml); however, Cry30Ba crystals were not toxic.
Palavras-Chave: Gene expression; Biological control; Microorganisms; Proteins; Pest control; Aquatic insects; Toxicity tests; Mortality; Crystal proteins; Crystals
Imprenta: Applied and Environmental Microbiology, v. 72, n. 8, p. 5673-5676, 2006.
Descritores: Aedes aegypti - Proteins
Data de publicação: 2006
