Cloning and expression of glucose regulated protein 78 (GRP78) in Fenneropenaeus chinensis
Autor(es): Luan, Wei; Li, Fuhua; Zhang, Jiquan; Wang, Bing; Xiang, Jianhai
Resumo: GRP78 (78kDa glucose-regulated protein), also known as BiP (immunoglobulin heavy-chain-binding protein), is an essential regulator of endoplasmic reticulum (ER) homeostasis because of its multiple functions in protein folding, ER calcium binding, and controlling of the activation of transmembrane ER stress sensors. In this report, we cloned the full length cDNA of GRP78 (FcGRP78) from Chinese shrimp Fenneropenaeus chinensis. This cDNA revealed a 2,325bp with 1,968bp open reading frame encoding 655 amino acids. This is the first reported GRP78 gene in Crustacea. The deduced amino acid sequence of FcGRP78 shared high identity with previously reported insect GRP78s: 86, 87 and 85% identity with GRP78s of Drosophila melanogaster, Aedes aegypti and Bombyx mori, respectively. Northern blot analysis shows that FcGRP78 is ubiquitously expressed in tissues of shrimp. Heat shock at 35C significantly enhanced the expression of FcGRP78 at the first hour, reached the maximum at 4h post heat shock, dropped after that and resumed to the normal level until 48h of post recovery at 25C. Additionally, differential expression of FcGRP78 was detected in haemocytes, hepatopancreas and lymphoid organ when shrimp were challenged by white spot syndrome virus (WSSV). We inferred that FcGRP78 may play important roles in chaperoning, protein folding and immune function of shrimp.
Palavras-Chave: Molecular biology; Symptoms; Calcium; Glucose; Proteins; Heat shock; Blood cells; Aquatic insects; Public health; white spot syndrome; Stress; Homeostasis; Endoplasmic reticulum; Protein folding; Hemocytes; Immune response; Hepatopancreas; Open reading frames; Amino acid sequence; Immunoglobulins; Aedes aegypti; Crustacea; Drosophila melanogaster; White spot syndrome virus; Fenneropenaeus chinensis; Bombyx mori
Imprenta: Molecular Biology Reports, v. 36, n. 2, p. 289-298, 2009.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Immune response ; Aedes aegypti - Proteins ; Aedes aegypti - Immune response ; Aedes aegypti - Virus ; Aedes aegypti - Immunology ; Aedes aegypti - Public health
Data de publicação: 2009
