Characterization of neuropeptide F and its receptor from the African malaria mosquito, Anopheles gambiae
Autor(es): Garczynski, S. F.; Crim, J. W.; Brown, M. R.
Resumo: The genome of Anopheles gambiae contains sequences encoding a neuropeptide F (Ang-NPF) and NPF receptor (Ang-NPFR) related to the neuropeptide Y signaling family. cDNAs for each were cloned and sequenced. Ang-NPFR was stably expressed for radioligand binding analysis. Ang-NPF exhibited high affinity (IC sub(50) similar to 3 nM) membrane binding; NPFs from Aedes aegypti (Aea-NPF) and Drosophila melanogaster (Drm-NPF) were less potent, with the rank order: Ang-NPF > Aea-NPF > Drm-NPF > Drm-NPF sub(8-36). RT- PCR analysis revealed Ang-NPF and Ang-NPFR transcripts in all life stages. Ang- NPF and Ang-NPFR may be strategically positioned for signaling in relation to nutritional status in the African malaria mosquito.
Palavras-Chave: Anopheles; Malaria; G protein-coupled receptor; Neuropeptide F; Neuropeptide Y
Imprenta: Peptides, v. 26, n. 1, p. 99-107, 2005.
Descritores: Aedes aegypti - RT-PCR
Data de publicação: 2005
