Biosynthesis of silver nanoparticles using Bacillus thuringiensis against dengue vector, Aedes aegypti (Diptera: Culicidae)

Autor(es): Banu, A.; Balasubramanian, C.; Moorthi, P.


Resumo: The present study reveals the larvicidal activity of silver nanoparticles (AgNPs) synthesized by Bacillus thuringiensis (Bt) against Aedes aegypti responsible for the diseases of public health importance. The Bt-AgNPs were characterized by using UVavisible spectrophotometer followed by scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy. A surface plasmon resonance spectrum of AgNps was obtained at 420 nm. The particle sizes were measured through SEM imaging ranging from 43.52 to 142.97 nm. The Bt-AgNPs has also given a characteristic peak at 3 keV in EDX image. Interestingly, the mortality rendered by Bt-AgNPs was comparatively high than that of the control against third-instar larvae of A. aegypti (LC50 0.10 ppm and LC90 0.39 ppm) in all the tested concentrations, viz. 0.03, 0.06, 0.09, 0.12, and 0.15 ppm. Hence, Bt-AgNPs would be significantly used as a potent mosquito larvicide against A. aegypti.


Palavras-Chave: Biological control; Particle size; Scanning electron microscopy; Vectors; Spectroscopy; Dengue; Nanoparticles; Silver; Larvicides; Biosynthesis; Microscopy; Spectrophotometers; Aedes egypti; Bacillus thuringiensis; Culicidae; Diptera


Imprenta: Parasitology Research, v. 113, p. 311-316, 2014.


Descritores: Aedes aegypti - Biosynthesis ; Aedes aegypti - Transmission ; Aedes aegypti - Larvicide ; Aedes aegypti - Dengue ; Aedes aegypti - Epidemiology ; Aedes aegypti - Public health


Data de publicação: 2014