Bioassay guided fractionation of Sphaeranthus indicus extract against mosquito vectors
Autor(es): Vidhya, P. T.; Mathew, Nisha
Resumo: This paper aims to at exploring the mosquitocidal potential of the extract of the medicinal plant Sphaeranthus indicus. The soxhlet extracts of flowers, leaves and arial parts of S. indicus with four solvents, viz., hexane, chloroform, ethyl acetate and methanol were screened against the larvae of the vector mosquitoes Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus. The commercially available synthetic insecticide temephos was used as a positive control. Bioassay guided fractionation was carried out for the hexane extract of the leaves of S. indicus using a silica gel column with eluents hexane followed by 5% and 10% ethyl acetate in hexane. Fractions were examined by thin layer chromatography using silica gel, 60 F254 plates using hexane-ethyl acetate, pooled the silmilar fractions and screened for mosquito larvicidal activity. The bioassay guided fractionation showed that the fractions F3-F7 eluted with hexane showed 100% mosquito larvicidal activity against all the three species of mosquitoes at 10 mg/L. FT-IR spectrum of this fraction revealed the presence of long chain alkanes.
Palavras-Chave: Sphaeranthus indicus; Larvicide; Mosquito; Vector; Anopheles; Culex; Aedes
Imprenta: International Journal of Pharmaceutical Sciences and Drug Research, v. 5, n. 9, p. 3965-3971, 2014.
Descritores: Aedes aegypti - Transmission
Data de publicação: 2014
