Autor(es): Ali, M. S.; Ravikumar, S.; Beula, J. M.

Resumo: Objective: To identify the larvieidal activity of the seagrass extracts. Methods: Seagrass extracts, Syringodium isoetifolium (S. isoetifolium), Cymodocea serrulata and Halophiia beccarii, were dissolved in DMSO to prepare a graded series of concentration. Batches of 25 early 4th instars larvae of Aedes aegypti (Ae. aegypli) were transferred to 250 mL, enamel bowl containing 199 mL of distilled water and 1 mL of plant extracts (0.01 mg - 0.1 mg). After 24 h the mortality rate was identified with the formulae [(% of test mortality - % of control mortality)/(100 - % of control mortality)] x 100. Each experiment was conducted with three replicates and a concurrent control group. A control group consisted of 1 mL of DMSO and 199 mL of distilled water only. Results: The root extract of S. isoetifolium showed maximum larvieidal activity with minimum concentration of extract of LC sub(50)=0.0604 plus or minus 0.0040), mu g/mL with lower confidence limit (LCL) - upper confidence limit (UCL) = (0.051-0.071) and LC sub(90)=0.0972 mu g/mL followed by leaf extract of S. isoetifolium showed LC sub(50)=(0.062 plus or minus 0.005) mu g/mL. The regression equation of root and leaf extract of S. isoetifolium for 4th instar larvae were Y=4.909 + 1.32x (R super(2)=0.909) and Y=2.066 + 1.21x (R super(2)=0.897) respectively. The results of ihe preliminary phytochemical constituents shows the presence of saponin, steroids, terpenoid, phenols, protein and sugars. Conclusions: From the present study the ethlanolic extracts of seagrass of S. isoetifolium possesses lead compound for development of larvicidal activity.

Palavras-Chave: Aedes aegypti; Bioinsecticides; Dengue fever; Larvicides; Seagrass; Syringodium isoetifolium; Cymodocea serrulata; Halophila beccarii; Mosquito; Extract

Imprenta: Asian Pacific Journal of Tropical Biomedicine, v. 2, n. 7, p. 570-573, 2012.

Descritores: Aedes aegypti - Proteins ; Aedes aegypti - Dengue

Data de publicação: 2012

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