A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus Stegomyia mosquitoes from the genus Aedes (Diptera:Culicidae) found in the Ryukyu Archipelago, Japan
Autor(es): Higa, Y.; Toma, T.; Tsuda, Y.; Miyagi, I.
Resumo: Internal transcribed spacer regions of ribosomal DNA were sequenced, and new species-specific primers were designed to simplify the molecular identification of five morphologically related subgenus Stegomyia mosquito species--Aedes aegypti, Ae. albopictus, Ae. riversi, Ae. flavopictus, and Ae. daitensis--found in the Ryukyu Archipelago, Japan. Each newly designed primer was able to amplify a species-specific fragment with a different size. Conditions for multiplex PCR were optimized to identify all five species in a single PCR. This method is a convenient tool for entomological field surveys, particularly in arbovirus endemic/epidemic areas where some of these species coexist.
Palavras-Chave: Endemic species; Epidemics; Infectious diseases; Nucleotide sequence; DNA; Archipelagoes; Polymerase chain reaction; Aquatic insects; Spacer region; Arbovirus
Imprenta: Japanese Journal of Infectious Diseases, v. 63, n. 5, p. 312-316, 2010.
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - DNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Molecular methods ; Aedes aegypti - Epidemic
Data de publicação: 2010
