A differentially displayed mRNA related to resistance to Bacillus thuringiensis israelensis of Aedes albopictus selected in vitro-activated CYT1AA6
Autor(es): Zhang, Lingling; Zhang, Qi; Huang, Enjiong; Li, Mingwei; Huang, Tianpei; Xu, Lei; Wu, Chanbiao; Guan, Xiong; Gelbic, Ivan
Resumo: The authors have compared the differential display of Aedes albopictus cells, both resistant and susceptible to Bacillus thuringiensis israelensis (Bti), using differentially displayed reverse transcription polymerase chain reaction. They found 1 band, about 200 base pairs long. After cloning and sequencing, the differentially expressed gene was similar to some partial messenger ribonucleic acid of Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae, rather than Aedes albopictus. This will be of some value for clarifying the mechanism of mosquito resistance to Bti products.
Palavras-Chave: Biological control; RNA; Nucleotide sequence; Polymerase chain reaction; Pest control; Aquatic insects; Control resistance; Reverse transcription; Culex quinquefasciatus; Bacillus thuringiensis israelensis; Aedes aegypti; Aedes albopictus; Anopheles gambiae; C6/36 cells
Imprenta: Journal of the American Mosquito Control Association, v. 28, n. 4, 327 p. 2012.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - RNA
Data de publicação: 2012
