Ornithine decarboxylase activity during the development of Anastrepha fraterculus (Diptera, Tephritidae)
Autor(es): Cardoso, VV; Prestes, PR; Caslali, EA; Moreira, JCF; Oliveira, AK
Resumo: Ornithine decarboxylase (ODC) (EC 4.1.1.17) is very important for polyamine biosynthesis, which is required for main biological events. In the present study, ODC activity was measured in samples of Anastrepha froterculu's egg, larva, pupa body and abdomen, adult body, ovaries, and fat body of young females, and in ovaries of mature flies. The kinetic parameters (K-m app and V-max) for ODC activity were determined for pupa, larva, and young ovary. ODC activity showed fluctuations during A. fraterculos life development. In its earlier stages, prior to emergence, the egg has high ODC-specific activity probably due to embryogenesis, which is characterized by a high rate of cell division. This enzyme activity is also significantly high in the ovary and fat body of young females possibly related to the increased oogenesis and vitellogenesis. The kinetic parameters (K-m app and V-max had great variation. Our results using GTP showed that the great variation in kinetic parameters can be accounted for by post-translational modifications. (C) 2004 Wiley-Liss, Inc.
Palavras-Chave: Anostrepha froterculus; Ornithine decarboxylose; Development; Reproductive kinetic parameters; GTP
Imprenta: Archives of Insect Biochemistry and Physiology , v. 57, n. 4, p. 151-159, 2004
Identificador do objeto digital: 10.1002/arch.20022
Descritores: Aedes aegypti - Biosynthesis
Data de publicação: 2004
