Autor(es): Rivero, Jose; Urdaneta, Ludmel; Zoghbi, Normig; Pernalete, Martha; Rubio-Palis, Yasmin; Herrera, Flor

Resumo: A modified technique to extract intact DNA from Aedes aegypti and Anopheles darlingi mosquitoes is presented. The samples were treated initially with proteinase K to digest all proteinaceous matter. For both vectors, the optimum temperature for the protease treatment was 55degreeC and the best pre-incubation time was 4 h. RNA contamination was eliminated with RNAse treatment. The purity of the DNA was high since the A260/A280 ratio averaged > 1.80 for all samples and the quantity of DNA extracted was > 1.6 times higher than that using the unmodified procedure. The DNA obtained displayed clear random amplified polymorphic DNA (RAPD) profiles, which indicates that it can be used for polymerase chain reaction (PCR)-based techniques.

Palavras-Chave: Anopheles darlingi; Aedes aegypti; DNA integrity; DNA extraction; Mosquito DNA

Imprenta: International Journal of Tropical Insect Science, v. 24, n. 3, p. 266-269, 2004

Identificador do objeto digital: 10.1079/IJT200430

Descritores: Aedes aegypti - Biochemistry

Data de publicação: 2004