Expression Strategy of Aedes albopictus Densovirus
Autor(es): Pham, Hanh T.; Jousset, Francoise-Xaviere; Perreault, Jonathan; Shike, Hiroko; Szelei, Jozsef; Bergoin, Max; Tijssen, Peter
Resumo: The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.
Palavras-Chave: Densonucleosis Virus; Genomic Organization; Nucleotide-Sequence; Promoter Element; Parvovirus Aapv;Aegypti Larvae; Tata Box; Dpe
Imprenta: Journal of Virology, v. 87, n. 17, p. 9928-9932, 2013
Identificador do objeto digital: 10.1128/JVI.01259-13
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Virus
Data de publicação: 2013
