Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus
Autor(es): Armengol, G; Guevara, OE; Orduz, S; Crickmore, N
Resumo: A mosquitocidal aquatic bacterium has been developed by introducing an operon containing the cry11Aa, and p20 genes from Bacillus thuringiensis subsp. israelensis (Bti) into the gram-negative aquatic bacterium Asticcacaulis excentricus. After transformation, the cry11Aa gene was successfully expressed in recombinant A. excentricus under the tac promoter, at the level of 0.04 pg/cell. The recombinant bacteria were toxic to Aedes aegypti larvae with an LC50 of 6.83 x 10(5) cells/mL. We believe that these bacteria may have potential as genetically engineered microorganisms for the control of mosquito larvae.
Palavras-Chave: Subsp Israelensis; Caulobacter-Crescentus; Biological-Control; Gene-Expression; Strain; Proteins;Crystal
Imprenta: Current Microbiology, v. 51, n. 6, p. 430-433, 2005
Identificador do objeto digital: 10.1007/s00284-005-0166-z
Descritores: Aedes aegypti - Cell
Data de publicação: 2005
