Autor(es): Effio, PC; Folgueras-Flatschart, AV; Montor, WR; Pernasetti, FM; Pueyo, MT; Sogayar, MC

Resumo: The Anopheles merus ( Diptera, Nematocera, Culicoidea) alpha-amylase gene (AmerAmy, GenBank Accession Number U01210) was amplified with its own or with the Zabrotes subfasciatus alpha-amylase signal peptide (ZsAmerAmy, GenBank Accession Number AY270183) by PCR, using designed primers. The AmerAmy gene was sequenced from its promotor to the TGA codon. As a positive control, the Z. subfasciatus alpha-amylase gene with its own signal peptide (ZsAmy, GenBank Accession Number AF255722) was also amplified by PCR. These three sequences were inserted into the baculovirus genome using the Bac-to-Bac(TM) system. Recombinant baculovirus preparations were used to infect Sf9 Spodoptera frugiperda insect cells. The A. merus alpha-amylase was successfully expressed as an active enzyme detected mainly in cell culture supernatants.

Palavras-Chave: Alpha Amylase; Anopheles Merus; Expression; Baculovirus/Spodoptera Frugiperda

Imprenta: Insect Molecular Biology, v. 12, n. 5, p. 415-425, 2003

Identificador do objeto digital: 10.1046/j.1365-2583.2003.00423.x

Descritores: Aedes aegypti - Genome

Data de publicação: 2003

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