Autor(es): Sazhenskiy, Vladislav; Zaritsky, Arieh; Itsko, Mark

Resumo: The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.

Palavras-Chave: Delta-Endotoxin; Antibacterial Activity; Crystal Protein; Invivo; Toxin; Cells; Gene; Sequence; Invitro;Cloning

Imprenta: Applied and Environmental Microbiology, v. 76, n. 10, p. 3409-3411, 2010

Identificador do objeto digital: 10.1128/AEM.03068-09

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Larvicide

Data de publicação: 2010

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