Autor(es): Tiawshisup, Sonthaya; Rattanakampol, Pichanan; Navavichit, Warritar; Ratpiyapaporn, Hemmarat

Resumo: This study was conducted to investigate Chikungunya virus (CHIKV) infection in mammal and avian by using mice and baby chickens as model animals. Thailand 2010 and Ross/186 strain (reference strain) were used in this study. 10(4), 10(6) and 10(8) CID50 of CHIKV were inoculated to four- and six-week-old mice. Blood was collected and tested for virus for seven days. 10(8) CID50 of CHIKV was inoculated to two-week-old mice. Blood was collected and tested for virus for five days. For the baby chickens, 10(2), 10(4), 10(6) and 10(8) CID50 of CHIKV were inoculated to five-day-old baby chickens. Blood was collected and tested for virus for seven days. Serum samples were tested for CHIKV by reverse transcription polymerase chain reaction. CHIKV was detected in two- and four-week-old mice that Were inoculated with 10(8) CID50 of CHIKV. The percentages of Thailand 2010 strain of CHIKV infection in four-week-old mice Were 80, 80, and 60% on days 1, 2, and 3 post inoculation (PI), respectively. The percentages of Ross/186 strain of CHIKV infection in four-week-old mice were 60, 100, and 60% on days 1, 2, and 3 PI, respectively. The percentages of Thailand 2010 strain of CHIKV infection in two-week-old mice were 90, 100, and 67% on days 1, 2, and 3 PI, respectively. The percentages of Ross/186 strain of CHIKV infection in two-week-old mice were 1.00, 100, 50, 83, and 100% on days 1, 2, 3, 4, and 5 PI, respectively. No virus was detected in any 6-week-old mice and baby chickens.

Palavras-Chave: Baby Chicken; Chikungunya Virus; Infection; Mice; Thailand

Imprenta: Thai Journal of Veterinary Medicine, v. 42, n. 3, p. 353-358, 2012

Descritores: Aedes aegypti - Chikungunya Fever

Data de Publicação: 2012