Evaluation of mosquito densoviruses for controlling Aedes aegypti (Diptera : Culicidae): Variation in efficiency due to virus strain and geographic origin of mosquitoes
Autor(es): Hirunkanokpun, Supanee; Carlson, Jonathan O.; Kittayapong, Pattamaporn
Resumo: Four mosquito densovirus strains were assayed for mortality and infectivity against Aedes aegypti larvae from different geographic regions. The viral titers were quantified by real-time PCR using TaqMan technology. First-instar larvae were exposed to the same titer of each densovirus strain for 48 hours. All strains of densoviruses exhibited larvicidal activity and caused more than 80% mortality and infectivity in the three mosquito strains. Aa/DNV-exposed larvae had the highest mortality rate. The mean time to death of Aa/DNV-exposed larvae was shorter than other DNVs-exposed larvae. We can conclude that different densovirus strains exhibit some variations in their pathogenicity to different populations of Ae. aegypti mosquitoes. A few mosquitoes from Chachoengsao and Bangkok exposed to AeDNV and A ThDNV survived to the adult stage to lay eggs and showed 22% to 50% vertical transmission in the F1 generation. Phylogenetic analysis of four densovirus strains indicated that mosquito densoviruses are separated into two distinct clades.
Palavras-Chave: Albopictus parvovirus aapv; Cell-line; Larvae; Infection; Densonucleosis; Expression; Aednv; Pathogenicity; Thailand
Imprenta: American Society of Tropical Medicine and Hygiene, v. 78, n. 5, p. 784-790, 2008
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Real Time PCR
Data de publicação: 2008
