Establishment and characterization of a cell line from the mosquito Culex tritaeniorhynchus (Diptera: Culicidae)
Autor(es): Kuwata, Ryusei; Hoshino, Keita; Isawa, Haruhiko; Tsuda, Yoshio; Tajima, Shigeru; Sasaki, Toshinori; Takasaki, Tomohiko; Kobayashi, Mutsuo; Sawabe, Kyoko
Resumo: We established a continuous cell line from the embryo of the mosquito Culex tritaeniorhynchus Giles (Diptera: Culicidae), a known major vector of the Japanese encephalitis virus (family Flaviviridae, genus Flavivirus) in Asia. The cell line, designated NIID-CTR, was serially subcultured in VP-12 medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS). It continued to grow for more than 60 passages over a 750-d period. The NIID-CTR cell line mainly comprised two morphologically distinct types of cells with adhesive properties: spindle-shaped and round cells. Most of the NIID-CTR cells at the 45th passage were diploid (2n = 6). The growth kinetics of the NIID-CTR cells was significantly affected by the FBS concentration in the medium. The population doubling time of the NIID-CTR cells was 20 h in the presence of 10 % FBS and 76 h in its absence. The DNA sequence of the mitochondrial cytochrome oxidase I gene confirmed that the NIID-CTR cell line was derived from C. tritaeniorhynchus. The cells were highly susceptible to Japanese encephalitis and Dengue viruses, thus providing a valuable tool for the study of mosquito-borne flaviviruses.
Palavras-Chave: Mosquito cell line; Japanese encephalitis virus; Dengue virus; Flaviviridae; Arbovirus
Imprenta: In Vitro Cellular & Developmental Biology-Animal, v. 48, n. 6, p. 369-376, 2012
Identificador do objeto digital: 10.1007/s11626-012-9520-1
Descritores: Aedes aegypti - Arbovirus ; Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Flaviviridae ; Aedes aegypti - RNA
Data de publicação: 2012
