Antennal expressed genes of the yellow fever mosquito (Aedes aegypti L.); characterization of odorant-binding protein 10 and takeout
Autor(es): Bohbot, J; Vogt, RG
Resumo: A small cDNA library was Constructed from antennae of 100 adult male Aedes aeqypti yellow fever mosquitoes. Sequencing of 80 clones identified 49 unique gene products, including a member of the Odorant Binding Protein family (Aaeg-OBP10), a homologue of Takeout (Aaeg-TO), and transposable elements of the LINE, SINE and MITE classes. Aaeq-OBP10 encodes a 140 amino acid protein including a predicted 25 amino acid signal peptide. Aaeg-OBP10 expression was adult male enriched, increased with adult age. and greatest in antennae and wings but also present in maxillary palps, proboscis and leg. Aaeg-OBP10 is a likely orthologue of Agam-OBP10 of the malaria mosquito Anopheles gambiae and shares significant similarity with members of the OBP56 gene cluster of Drosophila melanogaster. These OBP genes may represent a unified class of OBPs with unique roles in chemodetection; the expression pattern of Aaeg-OBP10 Suggests it may play a role in adult male chemosensory behavior. Aaeg-TO encodes a 248 amino acid protein including a predicted 22 amino acid signal peptide. Aaeg-TO is homologous with the circadian/feeding regulated D. melanogaster Takeout protein (Dmel-TO) and a subclass Of Juvenile Hormone Binding Proteins (JHBP) characterized by Moling from Manduca sexta; both Dmel-TO and Moling are sensitive to feeding, suggesting Aaeg-TO might regulate the antennal response to food, host or pheromonal odors in a JH sensitive manner. Aaeg-TO was used to identify 25 D. melanogaster and 13 A. gambiae homologues by Blast analysis Suggesting these may comprise a relatively large class Of protein involved in the hormonal regulation of behavior. (C) 2005 Elsevier Ltd. All rights reserved.
Palavras-Chave: Antenna; Olfaction; Gustation; OBP; Moling; Antenna; JHBP; Real-time PCR
Imprenta: Insect Biochemistry and Molecular Biology, v. 35, n. 9, p. 961-979, 2005
Identificador do objeto digital: 10.1016/j.ibmb.2005.03.010
Descritores: Aedes aegypti - Real Time PCR
Data de publicação: 2005
