Analyzing ligation mixtures using a PCR based method
Autor(es): Chandra, PK; Wikel, SK
Resumo: We have developed a simple and effective method (Lig-PCR) for monitoring ligation reactions using PCR and primers that are common to many cloning vectors. Ligation mixtures can directly be used as templates and the results can be analyzed by conventional gel electrophoresis. The PCR products are representative of the recombinant molecules created during ligation and the corresponding transformants. Orientation of inserts can also be determined using an internal primer. The usefulness of this method has been demonstrated using ligation mixtures of two cDNA's derived from the salivary glands of Aedes aegypti mosquitoes. The method described here is sensitive and easy to perform compared to currently available methods.
Palavras-Chave: Ligation; Nucleic acids; Polymerase chain reaction
Imprenta: Biological Procedures Online, v. 7, n. 1, p. 93-100, 2005
Identificador do objeto digital: 10.1251/bpo108
Descritores: Aedes aegypti - DNA
Data de publicação: 2005
