Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship
Autor(es): Dillon, Rod J.; Ivens, Al C.; Churcher, Carol; Holroyd, Nancy; Quail, Michael A.; Rogers, Matthew E.; Soares, M. Bento; Bonaldo, Maria F.; Casavant, Thomas L.; Lehane, Mike J.; Bates, Paul A.
Resumo: An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, < 4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (pelitrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signating pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania. (c) 2006 Elsevier Inc. All rights reserved.
Palavras-Chave: Lutzomyia; Expressed sequence tag; Leishmania; Genomics; Immunity; Parasite; Midgut
Imprenta: Genomics, v. 88, n. 6, p. 831-840, 2006
Identificador do objeto digital: 10.1016/j.ygeno.2006.06.011
Descritores: Aedes aegypti - Immune response ; Aedes aegypti - Molecular structure
Data de publicação: 2006
