Autor(es): Castaneda, Nadia Y.; Castellanos, Jaime E.; Zapata, Angela C.; Bello, Felio

Resumo: Mosquito cell derived cultures are useful tools for arbovirus Isolation, Identification or characterization. For studying dengue (DENV) and yellow fever viruses (YFV) Aedes albopictus C6/36 or Aedes pseudoscutellaris AP-61 cell lines, are normally used. The Aedes aegpti AEGY-28 cell line was obtained from embryonic tissues and characterized previously by one of us. In order to evaluate its susceptibility to two Flavivirus, AEGY-28 cells were inoculated with different multiplicity of infection (MOI) with type 2 DENV (COL-789, MOI: 1 and 5) and YFV clinical isolates (V-341, MOI 0,02) then processed at different times post infection (p.i.). Immunostaining and fluorometric cell-ELISA were carried out to identify and quantify viral antigens. C6/36 and Vero cells were used as positive controls. Unexpectedly, immunoreactivity was not found in inoculated AEGY-28 cells, even in higher MOI or late times p.i., therefore antigen quantification using fluorometric cell-ELISA were not plausible. Reverse transcriptase PCR with specific primers did not detect viral RNA in AEGY-28 inoculated cells. We can conclude that Aedes aegpti AEGY-28 cell line is not susceptible to dengue and yellow fever Flavivirus, a finding possibly related with the lacking of specific molecules at the plasma membrane or absence of cell machinery necessary for viral replication.

Palavras-Chave: Flavivirus, L¡nea celular, Virus dengue, Fiebre amarilla, Arbovirus, Infecci¢n

Imprenta: Acta Biologica Colombiana, v. 12, n. 2, p. 47-58, 2007

Descritores: Aedes aegypti - Arbovirus ; Aedes aegypti - RNA

Data de publicação: 2007

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