Aedes aegypti larvicide from the ethanolic extract of Piper nigrum black peppercorns
Autor(es): Santiago, Viviene S.; Grace Alvero, Rita; Villasenor, Irene M.
Resumo: Due to unavailability of a vaccine and a specific cure to dengue, the focus nowadays is to develop an effective vector control method against the female Aedes aegypti mosquito. This study aims to determine the larvicidal fractions from Piper nigrum ethanolic extracts (PnPcmE) and to elucidate the identity of the bioactive compounds that comprise these larvicidal fractions. Larvicidal assay was performed by subjecting 3rd to 4th A. aegypti instar larvae to PnPcmE of P. nigrum. The PnPcmE exhibited potential larvicidal activity having an LC50 of 7.1246 +/- 0.1304ppm (mean +/- Std error). Normal phase vacuum liquid chromatography of the PnPcmE was employed which resulted in five fractions, two of which showed larvicidal activity. The most active of the PnPcmE fractions is PnPcmE-1A, with an LC50 and LC90 of 1.7101 +/- 0.0491ppm and 3.7078ppm, respectively. Subsequent purification of PnPcmE-1A allowed the identification of the larvicidal compound as oleic acid.
Palavras-Chave: Larvicidal; Dengue; Aedes aegypti; Piper nigrum
Imprenta: Natural Product Research, v. 29, n. 5, p. 441-443, 2015
Identificador do objeto digital: 10.1080/14786419.2014.947490
Descritores: Aedes aegypti - Larvicide ; Aedes aegypti - Vaccine
Data de publicação: 2015
