Adaptation of an Aedes aegypti Mosquito Cell Line to Growth at 15 øC and its Response to Infection by Sindbis Virus
Autor(es): PELEG, J; PECHT, M
Resumo: Aedes aegyptiÿmosquito cells, usually cultured at 28 to 30 øC, were adapted to grow at 15øC. They were designatedÿA. aegyptiÿ(c) cells, and had an estimated doubling time of 10 days. Sindbis virus (SV) replicated in these cells to peak titres of over 1.0 ? 109ÿp.f.u./ml 8 to 10 days after inoculation. These, or about 10-fold lower titres, continued to be produced over a 130 day test period without causing visible cell damage. Continuous virus proliferation and the yield of uniformly large plaque forming progeny viruses are the two most important features which differentiate infection with this virus inÿA. aegyptiÿ(c) cells from that ofÿA. aegyptiÿcells grown at 28 øC (Peleg & Stollar, 1974). Absence of homologous interferenceÿvis-?-visÿcell-virus coexistence suggests that homologous interference is not a prerequisite for maintaining cell-virus coexistence. Preinoculation ofÿA. aegyptiÿ(c) cultures with a small plaque forming Sindbis virus (SV-S) leads, under certain conditions, to the establishment of homologous interference.
Imprenta: Journal of General Virology, v. 38, p. 231-239, 1978
Identificador do objeto digital: 10.1099/0022-1317-38-2-231
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Virus
Data de publicação: 1978
