Autor(es): McIntosh, AH; Grasela, JJ; Popham, HJR

Resumo: Insect cell lines from Arthropoda represented by Lepidoptera, Coleoptera, Diptera, and Homoptera were evaluated for their ability to support replication of AcMNPV. In addition, some of the cell tines that were refractive to AeMNPV were tested with AeMNPV hsp70 Red, a recombinant carrying the red fluorescent protein (RFP) gene, for their ability to express this protein after inoculation. Of the 10 lepidopteran cell lines tested, only three cell lines from Helicoverpa zea (BCIRL-HZ-AMJ), Lymantria dispar (IPLB-LD 65), and Cydia pomonella (CP-169) failed to support detectable viral replication as measured by tissue culture infectious dose 50 (TCID50) assay. Heliothis virescens (BCIRL-HV-AMI) produced the highest viral titer of 2.3 +/- 0.1. X 10(7) TCID50/ml followed by Heliothis subflexa (BCIRL-HS-AMI) at 4.7 +/- 0.1 X 10(6) TCID50/ml and Spodoptera frugiperda (IPLB-SF21) at 4.1 +/- 0.1 X 10(6) TCID50/ml. None of the coleopteran, dipteran, or homopteran cell lines supported AcMNPV replication. However, when studies were performed using AeMNPV hsp70 Red, the dipteran cell lines Aedes aegypti (ATC-10) and Drosophila inelanogaster (line 2), both expressed the RFP as well as the refractive lepidopteran cell lines from H. zea and L. dispar. No RFP expression was observed in any of the coleopteran or homopteran cell lines. Cell lines refractive to AeMNPV did not appear to be adversely affected by the virus, as judged by their ability to multiply, nor was there any indication of induced apoptosis, as assessed by deoxyribonucleic acid fragmentation profiles or cell blebbing, or both.

Palavras-Chave: Baculovirus; Gene expression; Insect cell lines; Red fluorescent protein

Imprenta: In Vitro Cellular & Developmental Biology-Animal, v. 41, n. 8, p. 298-304, 2005

Identificador do objeto digital: 10.1290/0412083R.1

Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA

Data de publicação: 2005

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