A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
Autor(es): Campos, J; Andrade, CFS; Recco-Pimentel, SM
Resumo: Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20degreesC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes.
Palavras-Chave: Cytology; Mosquito; Vector
Imprenta: Memorias do Instituto Oswaldo Cruz, v. 98, n. 3, p. 387-390, 2003
Identificador do objeto digital: 10.1590/S0074-02762003000300017
Descritores: Aedes aegypti - DNA
Data de publicação: 2003
