A tapeworm molecule manipulates vitellogenin expression in the beetle Tenebrio molitor
Autor(es): Warr, E.; Meredith, J. M.; Nimmo, D. D.; Basu, S.; Hurd, H.; Eggleston, P.
Resumo: Metacestodes of Hymenolepis diminuta secrete a molecule that decreases vitellogenin (Vg) synthesis in the beetle host, Tenebrio molitor. The 5608 bp T. molitor Vg cDNA represents a single-copy gene encoding a single open reading frame of 1821 amino acids with a predicted molecular mass of 206 kDa. Northern blot analysis revealed detectable levels of transcripts only in adult females. In vivo, Vg mRNA abundance was significantly higher in fat bodies from infected females compared with control females at all but the earliest time point. In vitro, Vg mRNA abundance was significantly increased in fat bodies incubated with live stage I-II parasites. The apparent conflict between increased Vg mRNA abundance and decreased Vg protein in fat bodies from infected females is discussed.
Palavras-Chave: Tenebrio molitor; Hymenolepis diminuta; Vitellogenin; mRNA abundance
Imprenta: Insect Molecular Biology, v. 15, n. 4, p. 497-505, 2006
Identificador do objeto digital: 10.1111/j.1365-2583.2006.00663.x
Descritores: Aedes aegypti - Protein synthesis ; Aedes aegypti - RNA
Data de publicação: 2006
