A single amino acid change in the E2 glycoprotein of Venezuelan equine encephalitis virus affects replication and dissemination in Aedes aegypti mosquitoes
Autor(es): WOODWARD, TM; MILLER, BR; BEATY, BJ; TRENT, DW; ROEHRIG, JT
Resumo: Four monoclonal antibody-resistant variants (MARVs) of Venezuelan equine encephalitis (VEE) virus were used to study mosquito-virus interactions. In vitro experiments using an Aedes albopictus cell line, C6/36, demonstrated that an amino acid change in the glycoprotein E2h, epitope (MARV 1A3B-7) decreased virus growth when compared with the wild-type, Trinidad donkey virus, and its vaccine derivative, TC-83. The MARVs replicated as efficiently as the parent virus when inoculated into Aedes aegypti mosquitoes, but MARV 1A3B-7 was restricted in its ability to infect and disseminate from the midgut following oral infection. These results demonstrate that a single amino acid change in the E2 glycoprotein can affect the ability of VEE virus to replicate and disseminate in Ae. aegypti mosquitoes.
Palavras-Chave: Monoclonal - Antibodies; Structural proteins; Lacrosse virus; Sindbis virus; Vector; Triseriatus; E2-Glycoprotein; Bunyavirus; Infection; Fusion
Imprenta: Journal of General Virology, v. 72, p. 2431-2435, 1991
Identificador do objeto digital: 10.1099/0022-1317-72-10-2431
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Virus
Data de publicação: 1991
