A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing
Autor(es)Iturbe-Ormaetxe, Inaki; Woolfit, Megan; Rances, Edwige; Duplouy, Anne; O'Neill, Scott L.
ResumoMost genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects. (C) 2010 Elsevier B.V. All rights reserved.
Palavras-ChaveWolbachia pipientis; Purification; Genomics; Symbiosis
ImprentaJournal of Microbiological Methods, v. 84, n. 1, p. 134-136, 2011
Identificador do Objeto Digital10.1016/j.mimet.2010.10.019
DescritoresAedes aegypti - Biochemistry ; Aedes aegypti - DNA ; Aedes aegypti - Genome
Data de Publicação:2011
