A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing
Autor(es): Iturbe-Ormaetxe, Inaki; Woolfit, Megan; Rances, Edwige; Duplouy, Anne; O'Neill, Scott L.
Resumo: Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects. (C) 2010 Elsevier B.V. All rights reserved.
Palavras-Chave: Wolbachia pipientis; Purification; Genomics; Symbiosis
Imprenta: Journal of Microbiological Methods, v. 84, n. 1, p. 134-136, 2011
Identificador do objeto digital: 10.1016/j.mimet.2010.10.019
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - DNA ; Aedes aegypti - Genome
Data de publicação: 2011
