Autor(es): Gu, Jin-Bao; Dong, Yun-Qiao; Peng, Hong-Juan; Chen, Xiao-Guang

Resumo: The Aedes aegyptt densovirus (AeDNV) has previously shown potential in mosquito control To improve its efficacy as a hopesticide, the gene for an excitatory insect-specific toxin from Buthus martensu Karsch (BmK IT!) was inserted into the AeDNV genome and cloned into pUCA plasmid The coding sequence for green fluorescent protein was hgated to the C-terminus of the BmK ITI gene as a screening marker Recombinant and helper plasmids were cotransfected into C6/36 cells, wild-type viruses were the controls The recombinant viruses were identified and quantified by real-time polymerase chain reaction and exposed to Ae alboptctus larvae for the evaluation of its bioinsecticidal activity LT(50), and LD(50) bioassays showed that the recombinant AeDNV had stronger and faster pathogenic effects on Ae albopictus than the wild-type virus This is the first report on the recombinant AeDNA containing the insect-specific toxin. BmK IT I which may be used to develop a novel type of insecticide

Palavras-Chave: Periplaneta - Fuliginosa densovirus; Nonstructural protein; Genetic manipulation; Densonucleosis virus; Aegypti mosquitos; Minute virus; Expression; Neurotoxin; Cells; Identification

Imprenta: American Journal of Tropical Medicine and Hygiene, v. 83, n. 3, p. 614-623, 2010

Identificador do objeto digital: 10.4269/ajtmh.2010.10-0074

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Molecular screening ; Aedes aegypti - Public health

Data de publicação: 2010

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