A quantitative in vitro assay for chemical mosquito-deterrent activity without human blood cells
Autor(es): Klun, Jerome A.; Kramer, Matthew; Zhang, Aijun; Wang, Shifa; Debboun, Mustapha
Resumo: We report that an aqueous solution containing 10(-3) M adenosine triphosphate (ATP) and citrate-phosphate-dextrose-adenine (CPDA-1) can effectively replace transfusable human red blood cells in an in vitro Klun and DEbboun bioassay system for evaluating chemicals for mosquito feeding-deterrent activity, using either Aedes aegypti or Anopoheles stephensi. These species fed with similar avidity through collagen membrane covering aqueous 10(-3) M ATP plus CPDA-1 or red blood cells in CPDA-1 supplemented with ATP. In a 2nd experiment, we evaluated the feeding-deterrent activity of N,N-diethyl-3-methylbenzamide and a newly discovered natural product chemical, (-)-isolongifolenone, against these 2 mosquito species. We found that the feeding-deterrent efficacy of the 2 chemicals was similar whether the feeding stimulant was red blood cells supplemented with ATP or ATP alone with CPDA-1. Since the use of human red blood cells in bioassays raises important health and logistic issues, aqueous ATP with CPDA-1 is a reasonable alternative to human blood cells for routine in vitoro chemical screening.
Palavras-Chave: Aedes aegypti; Anopheles stephensi; N,N-diethyl-3-methylbenzamide; ATP; Mosquito biting; Isolongifolenone; CPDA-1
Imprenta: Journal of the American Mosquito Control Association, v. 24, n. 4, p. 508-512, 2008
Identificador do objeto digital: 10.2987/08-5755.1
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Molecular screening ; Aedes aegypti - Public health
Data de publicação: 2008
