A putative receptor for dengue virus in mosquito tissues: Localization of a 45-kDa glycoprotein
Autor(es): Mendoza, MY; Salas-Benito, JS; Lanz-Mendoza, H; Hernandez-Martinez, S; del Angel, RM
Resumo: Dengue virus (DENV) infects target cells by attaching to various cell receptors, many of which are still unknown. In C6/36 cells (Aedes albopictus cell line), DENV-4 bound to two glycoproteins of 40 and 45 kDa, located on the cell surface. Preincubation of cells with polyclonal antibody against the 45-kDa protein specifically blocked DENV-4 infection of C6/36 cells. The antibody and purified DENV-4 detected the 45-kDa molecule in total extracts from eggs, larvae, and pupae as well as from the midgut, ovary, and salivary glands from adult-stage Aedes aegypti mosquitoes, whereas in malphigian tubules it was absent. This suggests that the distribution of the 45-kDa protein correlates with tissue tropism of DENV infection in mosquitoes. The 45-kDa molecule was not detected in Anopheles albimanus mosquito. The relevance of our findings is discussed from the pathogenetic and vector competence viewpoints.
Palavras-Chave: Trans-ovarial transmission; Aedes aegypti; Envelope protein; Binding; Cells; Albopictus; Susceptibility; Infection; Model; Ph
Imprenta: American Journal of Tropical Medicine and Hygiene, v. 67, n. 1, p. 76-84, 2002
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Cell ; Aedes aegypti - RNA
Data de publicação: 2002
