A polymerase chain reaction-based diagnostic to identify larvae and eggs of container mosquito species from the Australian region
Autor(es): Beebe, Nigel W.; Whelan, Peter I.; Van den Hurk, Andrew F.; Ritchie, Scott A.; Corcoran, Sarah; Cooper, Robert D.
Resumo: Dengue outbreaks occur regularly in parts of northern Queensland, Australia, and there is Concern that these outbreaks may spread with the introduction and range expansion of the two main vectors Aedcs aegypti (L.) and Aedes albopictus (Skuse). Problems encountered in separating larvae of endemic and exotic container mosquito species resulted in the development of a polymerase chain reaction diagnostic procedure that uses a restriction enzyme to cut the internal transcribed spacer region 1 of the ribosomal DNA to separate Ae. aegypti and Ae. albopictus from a number of common local container mosquito species which can be used at any stage of the life cycle, including eggs up to 8 wk of age. Identification was possible using desiccated or alcohol-preserved specimens with a response time of < 24 h after receipt of the specimens.
Palavras-Chave: Polymerase chain reaction diagnostic; Container mosquito species; Internal transcribed spacer region 1; Aedes mosquitoes
Imprenta: Journal of Medical Entomology, v. 44, n. 2, p. 376-380, 2007
Identificador do objeto digital: 10.1603/0022-2585(2007)44[376:APCRDT]2.0.CO;2
Descritores: Aedes aegypti - Infectious diseases ; Aedes aegypti - Public health
Data de publicação: 2007
