A novel lectin with a fibrinogen-like domain and its potential involvement in the innate immune response of Armigeres subalbatus against bacteria
Autor(es): Wang, X; Rocheleau, TA; Fuchs, JF; Hillyer, JF; Chen, CC; Christensen, BM
Resumo: Mosquitoes have an efficient cellular innate immune response that includes phagocytosis of microbial pathogens and encapsulation of metozoan parasites. In this study, we describe a novel lectin in the mosquito, Armigeres subalbatus (aslectin or AL-1). The 1.27 kb cDNA clone for the AL-1 gene (AL-1) encodes a 279 deduced amino acid sequence that contains a C-terminal fibrinogen-like domain. AL-1 is transcribed in all life stages. AL-1 mainly exists in the haemolymph of adult female mosquitoes, and is upregulated following both Escherichia coli and Micrococcus luteus challenge. AL-1 specifically recognizes N-acetyl-D-glucosamine and is able to bind both E. coli and M. luteus. These results suggest that AL-1 might function as a pattern recognition receptor in the immune response in Ar. subalbatus.
Palavras-Chave: Armigeres subalbatus; Pattern recognition receptor; Mosquito; Lectin; Innate immunity; Haemocyte
Imprenta: Insect Molecular Biology, v. 13, n. 3, p. 273-282, 2004
Identificador do objeto digital: 10.1111/j.0962-1075.2004.00484.x
Descritores: Aedes aegypti - Immune response ; Aedes aegypti - Molecular structure
Data de publicação: 2004
