Autor(es): Meredith, J. M.; Munks, R. J. L.; Grail, W.; Hurd, H.; Eggleston, P.; Lehane, M. J.

Resumo: A comparative analysis identified key cis-acting regulatory elements responsible for the temporal control of mosquito Defensin gene expression. The promoters of Anopheles gambiae Defensin 1 and two isoforms of Aedes aegypti Defensin A are up-regulated by immune challenge. This stimulated activity depends upon a cluster of three NF-kappa B binding sites and closely associated C/EBP-like motifs, which function as a unit for optimal promoter activity. Binding of NF-kappa B and C/EBP like transcription factors is confirmed by electrophoretic mobility shift assay, including supershifts with antibodies to C/EBP. kappa B-like motifs are abundant within antimicrobial peptide gene promoters and most are very closely associated with putative C/EBP binding sites. This novel association between NF-kappa B and C/EBP binding sites may, therefore, be of widespread significance.

Palavras-Chave: Immune regulation; Defensin genes; Mosquito; NF-kappa B; C/EBP

Imprenta: Insect Molecular Biology, v. 15, n. 4, p. 393-401, 2006

Identificador do Objeto Digital: 10.1111/j.1365-2583.2006.00635.x

Descritores: Aedes aegypti - Immune response ; Aedes aegypti - Molecular structure ; Aedes aegypti - Immunology

Data de Publicação: 2006